Abstract: The refractory fine disseminated copper sulphide ore in Tibet contains 0.45% copper and 3.1% sulfur, and the oxidation rate of copper is 9.91%.The uneven distribution of chalcopyrited and some ores showed the micro-fine-grained make it difficult to liberate with gangne, and it is the important factor influencing copper recovery. The Cu-S bulk flotation, copper-sulphur separation did not be improved after the re-grinding of primary concentrate and desulfurization flowsheet is adopted. The reagent regime for the ore is that lime is as pH regulator, A4 and ammonium dibutyl dithiophosphate were used as collectors for copper mine, amyl xanthate was used as collector for pyrite and MIBC was used as foaming agent. The good technical index had been achieved: the copper concentrate with Cu grade of 25.32% and Cu recovery of 85.56%; Au grade of 21.02 g/t and Au recovery of 63.37%; Ag grade of 119.25 g/t and Au recovery of 80.53%. Meanwhile, a sulfur concentrate with S grade of 19.82% and S recovery of 78.20% was obtained, realizing comprehensive utilization of sulfur in the ore.