Abstract: In view of a high-grade secondary copper sulfide ore from Serbia, combined with ore properties and flotation process characteristics, a series of experiments on grinding fineness, regulator, depressant, collector species and their dosage, and regrinding fineness of rougher concentrate were carried out, and the optimum range of technological parameters was determined. The process flow of copper preferential flotation and regrinding and recleaning of rougher concentrate is adopted. Though the closed-circuit test of one roughing, two cleaning, and two scavening, the copper concentrate is obtained with yield of 8.3%, copper grade of 25.87% and recovery of 91.37%, the gold content of copper concentrate is 5.9 g/t, and the gold recovery rate is 28.15%. The copper and gold recovery indexes of the copper concentrate are within the prediction range of the process mineralogy. The recommended process is simple and the index is excellent, which provides strong data support for the mine development.