薛永萍, 肖春桥, 张琰图, 池汝安. 解钾JX-10菌的筛选及解钾工艺[J]. 矿产综合利用, 2023, 44(2): 17-23. DOI: 10.3969/j.issn.1000-6532.2023.02.004
    引用本文: 薛永萍, 肖春桥, 张琰图, 池汝安. 解钾JX-10菌的筛选及解钾工艺[J]. 矿产综合利用, 2023, 44(2): 17-23. DOI: 10.3969/j.issn.1000-6532.2023.02.004
    Xue Yongping, Xiao Chunqiao, Zhang Yantu, Chi Ru’an. Screening of Potassium Dissolving JX-10 Bacteria and Research on the Optimization of Potassium-dissolving[J]. Multipurpose Utilization of Mineral Resources, 2023, 44(2): 17-23. DOI: 10.3969/j.issn.1000-6532.2023.02.004
    Citation: Xue Yongping, Xiao Chunqiao, Zhang Yantu, Chi Ru’an. Screening of Potassium Dissolving JX-10 Bacteria and Research on the Optimization of Potassium-dissolving[J]. Multipurpose Utilization of Mineral Resources, 2023, 44(2): 17-23. DOI: 10.3969/j.issn.1000-6532.2023.02.004

    解钾JX-10菌的筛选及解钾工艺

    Screening of Potassium Dissolving JX-10 Bacteria and Research on the Optimization of Potassium-dissolving

    • 摘要: 本文从一钾长石矿区土壤中分离、筛选得到了6株高效解钾细菌。其中命名为JX-10的菌株分离于矿区茼蒿根系土壤,经基因测序鉴定为Bacillus sp.,与KT981886菌相似度高到99.72%。同时就培养时间、温度及转速等解钾工艺条件进行了探讨。实验结果表明,JX-10菌株具有分解钾长石矿物的能力。其较佳解钾工艺条件为:培养温度28 ℃、时间10 d,转速160 r/min,培养基pH值为 5.0,钾长石浓度2 g/L,粒度0.03 mm,接种量25%,硫酸铵浓度0.2 g/L。在较佳工艺条件下,溶液中可溶性钾离子含量可达23.32 mg/L,浸出率为8.36%。

       

      Abstract: In this study, six strains with potassium-dissolving ability were screened from the soils collected from a K-feldspar mining area . One of them, which named JX-10 strain was identified as a kind of bacterium, by 16 S rRNA gene sequencing. Simultaneously, the optimization of potassium-dissolving were also discussed. The results show that, the JX-10 strain had an obvious dissolution effect on K-feldspar. The optimum conditions for the JX-10 strain to remove potassium from K-feldspar were as follows: cultured at 28 ℃ for 10 days, pH value of the culture medium of 5.0, 60 mL medium in a 250 mL conical flask, and 160 r/min shaking speed on a rotary shaker. The K-feldspar concentration and granularity, inoculation volume, ammonium sulfate dose were 2 g/L, 0.03 mm, 25%, 0.2 g/L, respectively. Under the above conditions, the highest the potassium content and corrosion efficiency reach to 23.32 mg/L and 8.36%, respectively.

       

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