Abstract: Gold content of a low-grade gold ore in Guangxi is 1.29 g/t, the gangue minerals are mainly quartz, and the content of non-ferrous metals such as copper, lead, zinc and other harmful elements arsenic is extremely low. The cyanide gold leaching test was carried out on the ore, and the effects of grinding fineness, pH value of solution, cyanide dosage, stirring speed and leaching time on the gold leaching effect were investigated respectively; The leaching aids with better leaching effect were mixed in the same proportion, and three new leaching aids A, B and C were obtained; for a low-grade gold ore in Guangxi, a cyanide leaching aid leaching test was carried out. The results show that the sample fineness of -0.074 mm accounts for 93.27%, the pH value of the solution is 10.5, the dosage of potassium cyanide is 4 kg/t, the stirring speed is 1500 r/min, and the leaching time is 24 h, the gold leaching rate is 24 h. 92.58%; the amount of potassium cyanide was reduced to 3 kg/t, and the other conditions remained unchanged, after adding the new leaching aid A for 18 h, the leaching rate of gold could reach more than 93%. The addition of the new leaching aid effectively improves the leaching rate of gold, reduces the loss of cyanide by 25%, and shortens the leaching time by more than 6 h.