Abstract: This is an essay in the field of mineral processing engineering. Pyrite and arsenopyrite are common associated sulfides. Because their crystal structures and surface properties are very similar, their flotation separation has been an important research topic. In the flotation process of arsenic and sulfur minerals, the oxidation reaction is easy to occur in different degrees. The oxidation treatment technology is used to strengthen the oxidation difference between the two minerals and to change the inherent flotation behavior of the minerals, the separation of arsenopyrite from pyrite can be realized. Based on the self-crystal structure and surface properties of pyrite and arsenopyrite, the research status and progress of separation technology and mechanism of pyrite and arsenopyrite in oxidative flotation are introduced in detail, the research direction is analyzed and prospected, and it is hoped to provide some reference for the green and efficient flotation separation of arsenic and sulfur minerals.