Abstract: The gold minerals of a gold ore in Gansu are mainly natural gold，the main metal minerals are mainly pyrite, arsenopyrite, stibnite, limonite.24.88% of gold in submicroscopic or micro inclusions existes in the pyrite, arsenopyrite and other sulfide minerals，10.87% of the gold in form of micro inclusions hosted in quartz and limonite, antimony ore gangue minerals. Directed at a fine grained disseminated arsenic and antimony-containing refractory gold ore, through detailed research and test conditions for testing the structure of different processes used in ore flotation + regrinding cyanide leach beneficiation process, the gold concentrate with gold grade of 50.21g / t and total recovery of 78.81% was obtained, achieving a high gold recovery.